Call us 24/7: + 1(925)399 0085

Get your paper done by an expert

No matter what kind of academic paper you need, it is simple and secure to hire an essay writer for a price you can afford at StudyAcer. Save more time for yourself.

WhatsApp + 1(925)399 0085
$ 10
free
  • bibliography
  • title page
  • revisions
per page
19 k happy customers
4.7 out of 5 satisfaction rate
27 writers active

Although the base sequence of most human DNA is identical from one person to another, there are sufficient differences to…

Although the base sequence of most human DNA is identical from one person to another, there are sufficient differences to enable use of this information to identify individuals. These differences in the sequence of bases in DNA from one individual to another lead to differences in the distribution and frequency of restriction enzyme recognition sites. Thus, when an individual’s DNA is fragmented with a specific restriction enzyme, the number and length of the resulting fragments is unique (unless the individual has an identical twin). The different length DNA fragments are called restriction fragment length polymorphisms, or RFLPs (pronounced Ríf-lips) for short.

 

After studying Figure 1, answer the following questions.

 

1)      How many RFLPs are generated from homologue M using this restriction enzyme? from homologue P?

2)      Explain this difference.

3)      Are the DNA segments in the diagram an entire chromosome? an entire gene? Explain.

 

                                                                  Figure 1.

 

A.  A Restriction Enzyme and its Cleaving Sequence

Enayme:  SalI derived from Streptomyces albus. This enzyme cuts DNA between G and T on the upper strand, between T and G on the lower strand, and horizontally between the two cuts.

 

Cleavage Sequence: 

          5 G T C G A C 3                               5 G                                         T C G A C 3 

          3 C A G C T G 5                  3 C A G C T          and                         G5

 

B.  Restriction Site Differences Between Two Homologous Chromosomes Using SalI

                 Homologue M                                                         Homologue P

 

A G G C G T C G A C G A G T C G A C T          A A G G C G T C G A C G A T T C G A C T

T C C G C A G C T G C T  C A G C T G A           T T C C G C A G C T G C T A A G C T G A

 

PART V. SOLVING A CRIME USING DNA (RFLP) FINGERPRINTS

 

One evening the police responded to a report of gunshots. Arriving at the scene, the officers found a parked pickup truck. In the passenger seat was a deceased male with several gunshot wounds. Footprints were visible in a pool of blood outside the truck, but they were not clear enough to identify the type of footwear that made them. Homicide detectives have narrowed the field to three suspects.

 

Suspect A was arrested near the scene and acknowledged knowing the victim. He was wearing black work boots that appeared to have dried blood on the soles.

 

At the home of suspect B, investigators recovered a pair of tennis shoes that also appeared to have dried blood on the soles. Suspect B says she had a severe nosebleed, which may have resulted in blood on her shoes.

 

Suspect C was the roommate of the deceased. When officers went to the apartment to notify him of the death, suspect C was packing a suitcase.  He was wearing sneakers that appeared to have dried blood on the soles and sides. When questioned about the blood and the murder, suspect C had no comment.

 

All the samples were tested and identified as human blood. The homicide detectives have asked the molecular biology laboratory to analyze the blood evidence using DNA fingerprint analysis. You are the forensic scientist assigned to the case. Figure 2 shows the DNA profiles from all the blood samples.

 

1.      Note that the box under the first column in Figure 2 (blood of victim) contains the number 1. This is the first unique DNA profile found.

2.      Moving from left to right, examine each DNA profile.

If the new profile does not match any previously examined profile, enter a new number in the box under the column (2,3,4 etc.).

If the new DNA profile is an exact match of a previous profile, enter the number you gave to the profile that matches this new profile.

Continue with this process until you have examined all the DNA profiles in Figure 2.

 

Questions:

1)      How many unique profiles were found?                

 

2)      Did any profiles match the blood from suspect A’s shoes?             If so, list them:                      

 

3)      Did any profiles match the blood from suspect B’s shoes?             If so, list them:                        

 

4)      Did any profiles match the blood from suspect C’s shoes?              If so, list them:                     

 

5)      Did any profiles match the blood from the deceased male?             If so, list them:                     

 

6)      Did any profiles match the blood found on the ground outside the truck?           

       If so, list them:                   

 

7)      Do these results make any individual a more likely suspect that the others?                     

 

8)      Explain your answer, citing facts from the DNA analysis.

 

 

 

Blood of victim

Blood on ground at scene

Blood of suspect A

Blood of suspect B

Blood of suspect C

Blood from suspect A’s shoes

Blood from suspect B’s shoes

Blood from suspect C’s shoes

1

 

 

—–

 

—–

—–

 

 

2

—–

—–

 

 

 

 

 

—–

3

—–

—–

 

 

—–

 

 

—–

4

 

 

—–

 

 

—–

 

 

5

 

 

—–

—–

 

—–

—–

 

6

 

 

 

—–

—–

 

—–

 

7

—–

—–

 

—–

 

 

—–

—–

8

 

 

 

 

—–

 

 

 

9

 

 

—–

 

—–

—–

 

 

10

—–

—–

 

 

 

 

 

—–

11

 

 

 

—–

 

 

—–

 

12

 

 

—–

 

—–

—–

 

 

13

 

 

 

—–

 

 

—–

 

14

 

 

 

 

 

 

 

 

15

—–

—–

 

 

 

 

 

—–

16

—–

—–

 

 

 

 

 

—–

17

 

 

 

 

 

 

 

 

18

 

 

 

—–

 

 

—–

 

19

 

 

 

 

 

 

 

 

20

—–

—–

—–

 

 

—–

 

—–

 

1

 

 

 

 

 

 

 

                                    Figure 2. DNA Profiles – Homicide case AF205

Our guarantees

Study Acers provides students with tutoring and help them save time, and excel in their courses. Students LOVE us!No matter what kind of essay paper you need, it is simple and secure to hire an essay writer for a price you can afford at StudyAcers. Save more time for yourself. Delivering a high-quality product at a reasonable price is not enough anymore.
That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.

Money-back guarantee

You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.

Read more

Zero-plagiarism guarantee

Each paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.

Read more

Free-revision policy

Thanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.

Read more

Privacy policy

Your email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.

Read more

Fair-cooperation guarantee

By sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.

Read more

Our Homework Writing Disciplines

With a highly diverse team in almost all academic fields including: