Microbiology Review Questions

Week10ReviewSheet I

Exercise1-Disinfectants

1. Whatdoesbactericidalmean?Bacteriostatic?Virucidal?Fungistatic?

2. Whyarecontrolculturesnecessaryinevaluatingdisinfectants?

3. Whatfactorscaninfluencetheactivityofadisinfectant?

4. Whydomicroorganismsdifferintheirresponsetodisinfectants?

5. Whatmicroorganismsaremostsusceptibleto disinfectants?

EXERCISE2-AntimicrobialAgentSusceptibilityTestingand Resistance

1. Whatismeantbyantimicrobialresistance?Susceptibility?

2. Whyarepureculturesusedforantimicrobialsusceptibilitytesting?

3. Woulditbeacceptabletouseamixedcultureforthistest?Why?

4. Listthreefactorsthatcaninfluencetheaccuracyofthetest.

5. Whenperformingabrothdilutiontest,whyis itnecessaryto includea growthcontroltube?

Asterilitycontroltube?

EXERCISE3-TheEnterobacteriaceae(EntericBacilli)

1. Whatdoestheterm IMViCmean?

2. Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?

3. WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other

Enterobacteriaceae?

4. HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?

5. Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?

EXERCISE4â€“Streptococci,Pneumococci,and Enterococci

1. Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby

Gramstain.

2. Whattypeof hemolysisis producedbyS. pneumoniae?

3. HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?

4. Describethehemolysisproducedbyalpha–hemolytic,beta-hemolytic,andnonhemolytic streptococci.

5. Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?

EXERCISE5-UrineCultureTechniques

1. Whatis bacteriuria?Whenis itsignificant?

2. Howdomicroorganismsentertheurinarytract?

3. Whyisasepticurinecollectionimportantwhenculturesareordered?

4. Listfivebacteriathatcancauseurinarytractinfection.

5. Ifyoucounted20 coloniesfrom a0.01–mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?

Week10ReviewSheet II

EXERCISE1-SerodiagnosisofInfectiousDisease

1. Defineserumtiter.

2. Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?

3. Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?

4. Ina pairedserumsample,whattestresultsindicaterecentinfection?

5. Whatis ahumoralantibody?

EXERCISE2-CulturingMicroorganismsfromtheEnvironment

1. Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?

2. Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?

3. Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?

4. Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?

5. Whenandwhyis handwashingimportantinpatientcare?

6. Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?

EXERCISE3-Staphylococci

1. Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby

Gramstain.

2. Whatarethetwotypesofstaphylococcalcoagulase?

3. Whatis proteinA?Describeonemethodofdetectingit.

4. Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?

5. Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?

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Must have APA citation and include questions and answers.

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